The heat shock response (HSR) is the main pathway used by cells to counteract proteotoxicity.The inability of differentiated neurons to induce an HSR has been documented in primary neuronal cultures and has been proposed to play a critical role in ageing and neurodegeneration.However, this accepted dogma has not been demonstrated in vivo.We used op
The discontinuous spectral element method (DSEM) coupled with an element-by-element sweeping strategy is proposed as a solution to the radiative luau thank you cards transfer equation (RTE) for participating media in irregular geometries.In the solving processing, a spatial domain of RTE is discretized by unstructured meshes; an angular direction o
Rapid and highly efficient mating-type switching of Saccharomyces cerevisiae enables a wide variety of genetic manipulations, such as the construction of strains, for instance, isogenic haploid pairs of both mating-types, diploids and polyploids.We used the CRISPR/Cas9 system to generate a double-strand break at the MAT locus and, in a single cotra